Altered drug susceptibility during host adaptation of a <i>Plasmodium falciparum</i> strain in a non-human primate model

Infections with Plasmodium falciparum, the most pathogenic of the Plasmodium species affecting man, have been reduced in part due to artemisinin-based combination therapies. However, artemisinin resistant parasites have recently emerged in South-East Asia. Novel intervention strategies are therefore urgently needed to maintain the current momentum for control and elimination of this disease. In the present study we characterize the phenotypic and genetic properties of the multi drug resistant (MDR) P. falciparum Thai C2A parasite strain in the non-human Aotus primate model, and across multiple passages. Aotus infections with C2A failed to clear upon oral artesunate and mefloquine treatment alone or in combination, and ex vivo drug assays demonstrated reduction in drug susceptibility profiles in later Aotus passages. Further analysis revealed mutations in the pfcrt and pfdhfr loci and increased parasite multiplication rate (PMR) across passages, despite elevated pfmdr1 copy number. Altogether our experiments suggest alterations in parasite population structure and increased fitness during Aotus adaptation. We also present data of early treatment failures with an oral artemisinin combination therapy in a pre-artemisinin resistant P. falciparum Thai isolate in this animal model

(Dis) integrated valuation - Assessing the information gaps in ecosystem service appraisals for governance support

The operational challenges of integrated ecosystem service (ES) appraisals are determined by study purpose, system complexity and uncertainty, decision-makers' requirements for reliability and accuracy of methods, and approaches to stakeholder-science interaction in different decision contexts. To explore these factors we defined an information gap hypothesis, based on a theory of cumulative uncertainty in ES appraisals. When decision context requirements for accuracy and reliability increase, and the expected uncertainty of the ES appraisal methods also increases, the likelihood of methods being used is expected to drop, creating a potential information gap in governance. In order to test this information gap hypothesis, we evaluate 26 case studies and 80 ecosystem services appraisals in a large integrated EU research project. We find some support for a decreasing likelihood of ES appraisal methods coinciding with increasing accuracy and reliability requirements of the decision-support context, and with increasing uncertainty. We do not find that information costs are the explanation for this information gap, but rather that the research project interacted mostly with stakeholders outside the most decision-relevant contexts. The paper discusses how alternative definitions of integrated valuation can lead to different interpretations of decision-support information, and different governance approaches to dealing with uncertainty. (C) 2017 Elsevier B.V. All rights reserved.Peer reviewe

Integrated assessment and valuation of ecosystem services - Guidelines and experiences

EU FP7 OpenNESS project deliverable D33 & D4

Stakeholders' perspectives on the operationalisation of the ecosystem service concept : Results from 27 case studies

The ecosystem service (ES) concept is becoming mainstream in policy and planning, but operational influence on practice is seldom reported. Here, we report the practitioners' perspectives on the practical implementation of the ES concept in 27 case studies. A standardised anonymous survey (n = 246), was used, focusing on the science-practice interaction process, perceived impact and expected use of the case study assessments. Operationalisation of the concept was shown to achieve a gradual change in practices: 13% of the case studies reported a change in action (e.g. management or policy change), and a further 40% anticipated that a change would result from the work. To a large extent the impact was attributed to a well conducted science-practice interaction process (>70%). The main reported advantages of the concept included: increased concept awareness and communication; enhanced participation and collaboration; production of comprehensive science-based knowledge; and production of spatially referenced knowledge for input to planning (91% indicated they had acquired new knowledge). The limitations were mostly case-specific and centred on methodology, data, and challenges with result implementation. The survey highlighted the crucial role of communication, participation and collaboration across different stakeholders, to implement the ES concept and enhance the democratisation of nature and landscape planning. (C) 2017 Published by Elsevier B.V.Peer reviewe

Burden of Illness in Irritable Bowel Syndrome Comparing Rome I and Rome II Criteria

Objectives: To evaluate the burden of illness in irritable bowel syndrome (IBS), in terms of resource utilisation (direct and indirect) and health-related quality of life (HR-QOL), in individuals with IBS who meet Rome I and Rome II criteria. Methods: A cross-sectional study, carried out by personal interview, on a representative sample (n = 2000) of the Spanish population. Individuals with suspected IBS were identified via a screening question and subsequently given an epidemiological questionnaire to complete. The questionnaire collected information on IBS symptoms, resource utilisation, and HR-QOL [Medical Outcomes Study 36-item Short Form (SF-36)]. Results: Sixty-five individuals met Rome II criteria for IBS, while 146 individuals met exclusively Rome I criteria. Of Rome II individuals, 67.7% had consulted some type of healthcare professional in the previous 12 months, compared with only 41.8% of those individuals meeting exclusively Rome I criteria (pIrritable bowel syndrome, Pharmacoeconomics, Quality of life, Resource use

Clonal outbreak of <i>Plasmodium falciparum</i> infection in Eastern Panama

Identifying the source of resurgent parasites is paramount to a strategic, successful intervention for malaria elimination. Although the malaria incidence in Panama is low, a recent outbreak resulted in a 6-fold increase in reported cases. We hypothesized that parasites sampled from this epidemic might be related and exhibit a clonal population structure. We tested the genetic relatedness of parasites, using informative single-nucleotide polymorphisms and drug resistance loci. We found that parasites were clustered into 3 clonal subpopulations and were related to parasites from Colombia. Two clusters of Panamanian parasites shared identical drug resistance haplotypes, and all clusters shared a chloroquine-resistance genotype matching the pfcrt haplotype of Colombian origin. Our findings suggest these resurgent parasite populations are highly clonal and that the high clonality likely resulted from epidemic expansion of imported or vestigial cases. Malaria outbreak investigations that use genetic tools can illuminate potential sources of epidemic malaria and guide strategies to prevent further resurgence in areas where malaria has been eliminated

Altered drug susceptibility during host adaptation of a Plasmodium falciparum strain in a non-human primate model

Infections with Plasmodium falciparum, the most pathogenic of the Plasmodium species affecting man, have been reduced in part due to artemisinin-based combination therapies. However, artemisinin resistant parasites have recently emerged in South-East Asia. Novel intervention strategies are therefore urgently needed to maintain the current momentum for control and elimination of this disease. In the present study we characterize the phenotypic and genetic properties of the multi drug resistant (MDR) P. falciparum Thai C2A parasite strain in the non-human Aotus primate model, and across multiple passages. Aotus infections with C2A failed to clear upon oral artesunate and mefloquine treatment alone or in combination, and ex vivo drug assays demonstrated reduction in drug susceptibility profiles in later Aotus passages. Further analysis revealed mutations in the pfcrt and pfdhfr loci and increased parasite multiplication rate (PMR) across passages, despite elevated pfmdr1 copy number. Altogether our experiments suggest alterations in parasite population structure and increased fitness during Aotus adaptation. We also present data of early treatment failures with an oral artemisinin combination therapy in a pre-artemisinin resistant P. falciparum Thai isolate in this animal model

Modeling malaria genomics reveals transmission decline and rebound in Senegal

U radu se analizira uloga i funkcija elektroničkog marketinga u općenitom smislu te u različitim načinima poslovanja. Elektronički marketing obuhvaća širu sliku svih njegovih nižih područja koja se smatraju jednim od najmodernijih načina promocije i oglašavanja. Informacijske i internetske tehnologije se u današnje vrijeme koriste u poslovnoj praksi na sve poznate načine. Korištenje i značaj svih internetskih tehnologija i njenih mrežnih usluga osjetno varira prvenstveno prema području i djelatnosti kojem subjekt pripada. To se temelji na pretpostavki da razina iskorištenosti elektroničkog poslovanja značajno varira ovisno o području u kojem se subjekt nalazi. Ipak, gospodarsko područje i njegove specifičnosti nikako nisu prepreka u korištenju internetskih tehnologija i zbog toga je uspješnost i dalje značajna u korištenju elektroničkih resursa u poslovanju.The paper analyzes the function and role of eletronic marketing in a general sense and in different ways of business. Email marketing encompasses a broader picture of all its smaller areas, considered one of the most modern ways of promotion and advertising. Information and Internet technologies are nowadays used in business practice in every known way. The significance and utilization of Internet technologies and associated network services varies markedly primarily by area and activity. It is based on assumption that the level of implementation of e – commerce varies significantly depending on the area in which the observed business entity is located. However, the economis area and its specificities are by no means an obstacle to the use of Internet technologies and therefore the success is still significantly important to the use of electronic resources in business